热带病与寄生虫学

热带病与寄生虫学 ›› 2014, Vol. 12 ›› Issue (2): 89-91.doi: 10.3969/j.issn.1672-2302.2014.02.011

• 论著 • 上一篇    下一篇

改良加藤厚涂片法检查土源性线虫卵的检出效果

周莉,金伟*, 郭见多,朱磊,罗俊平,李启扬   

  1. 230061 合肥市，安徽省血吸虫病防治研究所;* 通讯作者
  • 出版日期:2014-06-10 发布日期:2014-08-13

The detective effect on the eggs of soil- transmitted nematodes by Kato- Katz technique in the single smear

Zhou Li, Jin Wei*, Guo Jianduo, Zhu Lei, Luo Junping, Li Qiyang.   

  1. Anhui Provinical Institute of Schistosomiasis Control, Hefei 230061, China. * Corresponding author.
  • Online:2014-06-10 Published:2014-08-13

被引次数

6

摘要： 目的 分析改良加藤厚涂片法（Kato-Katz）1 送3 检中单次涂片检出率规律，并探索单次涂片检出率与粪便中虫卵密度的相互关系。方法 2006~2010 年，每年9~11 月在安徽省蒙城县监测点采集3周岁以上常住居民的粪便，采用改良加藤厚涂片法（1 送3 检）检查蛔虫、鞭虫和钩虫卵；以片均虫卵数（eggs per slide，EPS）作为描述粪便虫卵密度的计算单位。对虫卵在3 张涂片中的各自出现概率进行分析和对比。结果 3 张涂片的虫卵出现率差异均无统计学意义。当EPS<2 时，单张涂片的虫卵平均出现率为52.26%；当EPS≥16 时，单张涂片虫卵出现率可达99.26%。结论 单张涂片的虫卵平均出现率与EPS 存在一定关联。

关键词: 土源性线虫, 改良加藤厚涂片法, 检出率, 粪便虫卵密度

Abstract:

Objective To analyze on the eggs of soil-transmitted nematodes by Kato-Katz technique in the single smear, and explore the interrelation between the detection rate of singe slide of Kato-Katz and the density of eggs in the stool samples. Methods The stool samples of the residents above three years old at Mengcheng County in Anhui were collected, and Ascaris lumbricoides, Trichuris trichiura and hookworms with modified Kato- Katz thick smear were examined once a year from September to November, 2006 to 2010. The number of eggs per slide was used to describe the density of eggs in the stool samples. The probability of the eggs appeared in the three smears was analyzed and compared. Results There was no statistical significance difference on the occurrence rate of eggs among the three slides. The occurrence rate of eggs was 52.26% in a single smear when the EPS less than 2, and 99.26% when the EPS was more than 16.Conclusion The occurrence rate of eggs in a singe slide was associated with EPS by Kato-Katz technique.

Key words: Soil-transmitted nematodes, Kato-Katz technique, Detective rate, Density of eggs in stool sample