热带病与寄生虫学

热带病与寄生虫学 ›› 2021, Vol. 19 ›› Issue (6): 348-350,357.

• 综述 • 上一篇    下一篇

小型节肢动物无形态损伤提取基因组 DNA 的研究进展

范海页,陈慧林,朱永航,王严,王若航,赵薇,陶香林,孙恩涛
  

  1. 皖南医学院检验学院,安徽 芜湖 241002
  • 出版日期:2021-12-20 发布日期:2021-12-31
  • 通信作者: 孙恩涛,E-mail:asdentao@ 126. com
  • 作者简介:范海页,女,本科在读,研究方向:媒介生物检验与检疫。 E-mail:fhy2743317586@ 163. com
  • 基金资助:
    国家自然科学基金项目(31870352);皖南医学院 2019 年青年卓越人才资助项目(WYQNYX201902);安徽省级大学生创新创业项目
    (S202010368034)

Advances ingenomic DNA extraction from microarthropods without morphological damage

FAN Hai-ye,CHEN Hui-lin,ZHU Yong-hang,WANG Yan,WANG Ruo-hang,ZHAO Wei,TAO Xiang-lin,SUN En-tao #br#   

  1. Medical Laboratory Science,Wannan Medical College,Wuhu 241002,Anhui Province,China
  • Online:2021-12-20 Published:2021-12-31

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摘要： DNA 提取是获取 DNA 条形码的关键步骤及进行后续相关研究的基础。 小型节肢动物因个体微小,常用的基因组 DNA 提取方法破坏了重要的外部形态特征,致使无法进行后续的形态学验证,而无形态损伤提取 DNA 的方法有效弥补前者的不足。 本文对小型节肢动物的无形态损伤提取基因组 DNA 进行综述,对比了小型节肢动物不同基因组 DNA 提取方法的优缺点,为进一步优化改进,探索更简单、高效的无形态损伤性 DNA 提取方法奠定基础。

关键词: DNA 条形码, 小型节肢动物, 无形态损伤性取样, 基因组 DNA 提取

Abstract: DNA extraction represents the key step for DNA barcoding and the basis of subsequent field study. Common genomic DNA extraction technique may result in damage to the important external morphological nature of microarthropods due
to their smaller size,which makes it impossible to further identify the morphology. However,a method of DNA extraction
without morphological damage can effectively make up for the deficiency of the former. This paper reviewed the genomic
DNA extraction technique without morphological damage to the microarthropods,and compared the advantages and disadvantages of different genomic DNA extraction procedures for microarthropods,with an attempt to lay a foundation for further optimization,improvement and exploration of a simpler and more efficient DNA extraction technique without morphological
damage.

Key words: DNA barcoding, Microarthropods, Sampling without morphological damage, Genomic DNA extraction

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