热带病与寄生虫学

热带病与寄生虫学 ›› 2023, Vol. 21 ›› Issue (1): 35-43.doi: 10.3969/j.issn.1672-2302.2023.01.007

• 论著 • 上一篇    下一篇

膳食结构对鞭虫感染人群肠道菌群的影响研究

张艺馨1(), 王龙江1, 刘建成2, 刘萍萍3, 王用斌1, 许艳1, 闫歌1, 卜秀芹1, 张佃波1, 李曰进1, 张本光1()   

  1. 1.山东省寄生虫病防治研究所，山东第一医科大学（山东省医学科学院），山东济宁272033
    2.临沂市疾病预防控制中心
    3.日照市岚山区疾病预防控制中心
  • 收稿日期:2023-01-06 出版日期:2023-02-20 发布日期:2023-02-28
  • 通信作者: 张本光 E-mail:benguangzhang@163.com
  • 作者简介:张艺馨，女，硕士在读，研究方向：寄生虫病与肠道菌群的关系。E-mail: 1367442659@qq.com
  • 基金资助:
    山东省医药卫生科技发展计划(202001050586);山东省自然科学基金项目(ZR2017YL005);山东第一医科大学学术提升计划(2019QL005);山东省医学科学院医药卫生科技创新工程

Effect of dietary structures on intestinal flora in population infected with Trichuris trichura

ZHANG Yi-xin1(), WANG Long-jiang1, LIU Jian-cheng2, LIU Ping-ping3, WANG Yong-bin1, XU Yan1, YAN Ge1, BU Xiu-qin1, ZHANG Dian-bo1, LI Yue-jin1, ZHANG Ben-guang1()   

  1. 1. Shandong Institute of Parasitic Disease Control, Shandong First Medical University (Shandong Academy of Medical Sciences), Jining 272033, Shandong Province, China
    2. Linyi Center for Disease Control and Prevention
    3. Disease Control and Prevention Center of Lanshan District, Rizhao City
  • Received:2023-01-06 Online:2023-02-20 Published:2023-02-28

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摘要：

目的 探讨不同膳食结构对鞭虫感染人群肠道菌群的影响，为研究鞭虫感染者肠道疾病与饮食关系奠定基础。方法 在山东省日照市岚山区5个乡镇选取34名鞭虫感染者，收集研究对象膳食资料，通过16S rDNA测序平台分析不同膳食结构下鞭虫感染人群肠道菌群多样性和物种组成的差异。结果 未发现膳食摄入量高低与肠道菌群α多样性或β多样性有关，但蔬菜、奶制品和肉制品三种膳食结构与鞭虫感染人群肠道菌群的丰度与组成有显著关联。不同蔬菜摄入量组在属水平上相对丰度变化差异有统计学意义的有3个菌属：每日摄入量<300 g的鞭虫感染组（PDV3组）狭义梭菌属1（Clostridium sensu stricto 1）相对丰度高于每日摄入量>500 g的鞭虫感染组（PDV1组）（t=2.211，P<0.05）；PDV3组瘤胃球菌属（Ruminococcus）相对丰度低于PDV1组（t=2.246，P<0.05）；PDV3组双歧杆菌属（Bifidobacterium）相对丰度低于每日摄入量300~500 g的鞭虫感染组（PDV2组）（t=2.610，P<0.05）。不同奶制品摄入量组在属水平上相对丰度变化差异有统计学意义的有3个菌属：每日摄入量300~500 g的鞭虫感染组（PDD2组）狭义梭菌属1相对丰度高于每日摄入量>500 g的鞭虫感染组（PDD1组）（t=3.025，P<0.05）；每日摄入量<300 g的鞭虫感染组（PDD3组）另枝菌属（Alistipes）相对丰度低于PDD1组（t=3.234，P<0.05）；PDD3组瘤胃球菌UCG-014属（Ruminococcaceae UCG-014）相对丰度低于PDD2组（t=2.255，P<0.05）。不同肉制品摄入量组在属水平上相对丰度变化差异有统计学意义的有3个菌属：每日摄入量120~200 g的鞭虫感染组（PDM2组）考拉杆菌属（Phascolarctobacterium）和瘤胃球菌属2（Ruminococcus 2）相对丰度低于PDM1组（t=2.672、2.731，P均<0.05）；每日摄入量<120 g的鞭虫感染组（PDM3组）小类杆菌属（Dialister）相对丰度低于PDM2组（t=2.402，P<0.05）。结论 不同膳食结构影响鞭虫感染人群的肠道菌群组成，提示应重视日常膳食摄入对鞭虫感染人群肠道疾病的调节作用，通过补充蔬菜、奶制品、肉制品来降低狭义梭菌属1等有害菌丰度，提高双歧杆菌属和另枝菌属等有益菌丰度，提高机体免疫力，减轻或避免鞭虫感染人群肠道炎症性疾病的发生发展。

关键词: 鞭虫, 膳食结构, 肠道菌群, 相对丰度, 多样性

Abstract:

Objective To investigate the effects of different dietary structures on the intestinal flora in population infected by Trichuris trichura (T. trichura) so as to lay a foundation for studying the relationship between intestinal diseases and diet in patients with T. trichura infection. Methods Thirty-four patients infected by T. trichura were recruited from five towns in Lanshan District, Rizhao City, Shandong Province, and their dietary information was collected and analyzed by 16S rDNA high-throughput sequencing to determine the differences in intestinal flora diversity and species composition among T. trichura infected populations with different dietary structures. Results No association was found between high dietary intake and gut flora Alpha diversity or Beta diversity. The three dietary structures of vegetables, dairy products and meat products were significantly associated with the abundance and composition of intestinal flora.There were three species with significant differences in the intake of different vegetables at the genus level. The relative abundance of Clostridium sensu stricto 1 in the PDV3 group with a daily intake of <300 g was higher than that in PDV1 group with a daily intake of >500 g, and the difference was statistically significant (t=2.211, P<0.05). The relative abundance of Ruminococcus in PDV3 group was significantly lower than that in PDV1 group (t=2.246, P<0.05), whereas the relative abundance of Bifidobacterium was lower in PDV3 group than in PDV2 group with daily intake of 300-500 g. The difference was significant (t=2.610, P<0.05). Three species were significantly different in the intake of different dairy products at the genus level. The abundance of Clostridium sensu stricto 1 in PDD2 group with daily intake of 300 g-500 g was higher than that in PDD1 group with daily intake of >500 g(t=3.025, P<0.05), contrarily, the abundance of the Alistipes in PDD3 group with daily intake <300 g was lower than that in PDD1 group (t=3.234, P<0.05). The abundance of Ruminococcaceae UCG-014 was significantly lower in PDD3 group than in PDD2 group (t=2.255, P<0.05). Three species with significant differences in the intake of different meat products at the genus level were observed. The abundance of the genus pair of Phascolarctobacterium and Ruminococcus 2 in PDM2 group with a daily intake of 120-200 g was lower than that in the PDM1 group, with significant difference between groups (t=2.672, P<0.05; t=2.731, P<0.05), yet the relative abundance of Dialister in PDM3 group with daily intake <120 g was lower than that in PDM2 group (t=2.402, P<0.05). Conclusion Different dietary structures affected the composition of intestinal flora in whipworm-infected populations. By supplementing the diet with vegetables, dairy products and meat products to reduce harmful bacteria such as Clostridium sensu stricto 1 and increasing beneficial bacteria such as Bifidobacterium and Alistipes can improve the immunity of the body and reduce or avoid the development of intestinal inflammatory diseases in people with T. trichura infection.

Key words: Trichuris trichura, Dietary structures, Intestinal flora, Relative abundance, Diversity

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