热带病与寄生虫学

热带病与寄生虫学 ›› 2015, Vol. 13 ›› Issue (2): 63-65.doi: 10.3969/j.issn.1672-2302.2015.02.001

• 论著 •    下一篇

贝类多糖对HepG2.2.15细胞JAK-STAT信号转导途径分子表达的影响

湛孝东,王克霞   

  1. 241002 安徽芜湖市，皖南医学院医学寄生虫学教研室（湛孝东），安徽理工大学医学院病原生物学教研室（王克霞）
  • 出版日期:2015-06-10 发布日期:2015-07-10
  • 基金资助:

    皖南医学院中青年科研基金项目（WK201209）

Effects of shellfish polysaccharide on the molecular expression of JAK-STAT transduction pathways in HepG2.2.15 cells

Zhan Xiaodong, Wang Kexia.   

  1. Department of Medical Parasitology, Wannan Medical College, Wuhu 241002, China
  • Online:2015-06-10 Published:2015-07-10

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被引次数

1

摘要： 【摘要】 目的  了解贝类多糖对HepG2.2.15细胞JAK-STAT信号转导途径分子表达的影响。 方法  用RT-PCR的方法检测经贝类多糖处理的HepG2.2.15细胞在不同时间段的STAT1和STAT2 mRNA表达的水平。 结果  RT-PCR结果显示经贝类多糖处理后HepG2.2.15细胞STAT1和STAT2 mRNA表达水平明显升高，与对照组比较差异有统计学意义（P<0.05），表明HepG2.2.15细胞对贝类多糖有应答。 结论  贝类多糖对HBV有一定的抑制作用，其作用机制可能与INF-α类似。

关键词: 贝类多糖, HepG2.2.15细胞, JAK-STAT信号通路

Abstract:

【Abstract】 Objective  To understand the influence of shellfish polysaccharide on the molecular expression of JAK-STAT transduction pathways in HepG2.2.15 cells. Methods  After treatment with shellfish polysaccharides, the expression levels of STAT1 and STAT2 mRNA in HepG2.2.15 cells in different phase were detected by reverse transcription polymerase chain reaction (RT-PCR). Results  RT-PCR results showed that the expression levels of the STAT1and STAT2 mRNAs in HepG2.2.15 cells treated with shellfish polysaccharides were significantly up-regulated as compared with the control group (P<0.05), which suggested the HepG2.2.15 could respond to the shellfish polysaccharides. Conclusion  The shellfish polysaccharide has certain inhibitory effect on hepatitis B virus(HBV), and its mechanism looks similar to the INF-α.

Key words: Shellfish polysaccharide, HepG2.2.15 cells, JAK-STAT transduction pathways